FastQCFastQC Report
Sun 14 Apr 2019
SRR6576585_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576585_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264247
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4880.18467570114324855No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3390.12828906288434686No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2750.10406929879998637No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2650.10028496066180506No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACT502.9996918E-547.0089954
GTATCAA7800.046.5119821
ATCAACG9500.037.6143153
AACGCAG9950.036.8512736
CAACGCA10100.036.3039745
TCAACGC10000.036.1969224
GTACATG18000.035.3370251
TACATGG17750.035.230312
ACATGGG17450.035.0276573
GTATTAA851.720404E-533.2583771
TCTATAC851.7451397E-533.18913
ACGCAGA11250.032.9982647
CGCAGAG11100.032.603678
TATCAAC11050.032.3380972
TCCTATA600.004147499331.345262
GTTATAC600.004147499331.345263
CATGGGG6900.031.3393274
GCAGAGT12150.029.7860689
GGGTACC2250.029.238977
ATGGGTA2750.029.0601065
Produced by FastQC (version 0.11.2)