FastQCFastQC Report
Sun 14 Apr 2019
SRR6576585_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576585_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264247
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5530.20927389904142718No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2860.10823207075198583No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2800.10596146786907704No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2650.10028496066180506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7250.042.148711
GTACATG19050.038.251251
TATTGGG500.001697521437.6096152
TACATGG19400.037.561152
CATGGGT3450.036.778094
ACATGGG19550.036.537713
ACTTATA550.00270983834.1905632
ATGGGAG4950.034.177625
TACGCTA1309.142241E-932.5344669
AACGCAG9350.032.167176
GGTACCT2050.032.093628
ATGGGTA2350.031.996075
CATGGGA11650.031.8673294
CAACGCA9150.031.8430845
ATCAACG9150.031.8430843
CTAGACA753.2711253E-431.3294874
GGGTACC2300.030.6484097
TCAACGC9600.030.350444
ATACGCT1401.8815626E-830.2105758
ATATACG1252.3540815E-730.0763076
Produced by FastQC (version 0.11.2)