FastQCFastQC Report
Sun 14 Apr 2019
SRR6576584_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576584_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104357
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1480.14182086491562618No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1070.10253265233764865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTACC504.5207526E-756.4046067
TGGGGGG602.5243935E-854.8378076
CACTAGA450.001009310741.7811852
TACATGG6150.040.5073662
GGTACCT704.561989E-640.3276638
CATGGGT1400.040.2894
GTACATG6300.039.5429081
ACATGGG6400.038.9250533
TGGGTAC757.374674E-637.6030656
GAATAGG801.14795275E-535.2528761
ATGGGGG959.33569E-734.6344035
CATGGGA3400.034.561644
ATGGGAG1800.033.9472165
TCCTAGC650.00609778328.9531978
ATGGGTA1005.2749834E-528.2023035
GTATCAA2900.027.553971
CATGGGG2400.027.4189034
GGTCAAT350.008826558526.85289834-35
GTACCTG1151.3558951E-424.5472769
TACTTTT2950.023.9002552
Produced by FastQC (version 0.11.2)