FastQCFastQC Report
Sun 14 Apr 2019
SRR6576584_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576584_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104357
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1560.14948685761376812No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGCG250.005223900556.3968354
GTACATG5250.037.597891
GATCAAC500.001695409837.597891
GTCCAGA500.001695409837.597891
TACATGG5600.036.087262
ACATGGG5950.034.7543563
CAAGTCA550.002706494234.17994
TCTAGGC702.1721795E-433.5695463
CATGGGC1001.3955432E-632.8981554
GGATAGA600.00414495531.3315731
ACTATGT600.00415469831.3165638
ATGGGTG953.721448E-529.6825455
ATCTAGG650.00613057628.9214532
GTAGAAC650.00613057628.9214533
GGTACCT650.006144960428.9075978
CATGGGT1503.63234E-828.1984184
CTTCTAA700.00880230926.8556371
ATGGGAT1101.0100516E-425.6349265
CATGGGG2051.0531949E-925.2180984
CATGGGA3350.023.849414
Produced by FastQC (version 0.11.2)