FastQCFastQC Report
Sun 14 Apr 2019
SRR6576583_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576583_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116802
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1520.13013475796647317No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG250.005225057756.3979455
CTATTAA601.5558344E-647.1597981
GTACATG6050.045.9905431
ATGGGTA954.2018655E-1044.5246935
GGGTACC954.2018655E-1044.5246937
TACATGG6800.042.8513832
ACATGGG6650.042.4044723
GTAGCAC450.001010395541.7762573
GGTACCT1101.7680577E-938.4531448
GTATCAA3250.037.7278371
ATGGGAG1800.036.5542265
CATGGGT1700.035.939874
AACGCAG3300.035.6047636
ATCAACG3450.035.4193
TGGGTAC1204.14002E-935.248726
TCAACGC3400.034.5575684
CAACGCA3450.034.056735
CATGGGA3750.033.8387684
CATGGGC1351.3062163E-831.3321934
TATCAAC3950.030.9355812
Produced by FastQC (version 0.11.2)