FastQCFastQC Report
Sun 14 Apr 2019
SRR6576583_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576583_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116802
Sequences flagged as poor quality0
Sequence length100
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1490.12756630879608227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTATA407.992034E-658.7462582
TATACAG500.001696306437.5976035
GTATCAA3000.036.0310361
ATGGGAG1601.8189894E-1235.2477535
CATGGGA3550.033.096484
GTACATG6350.031.8247431
TACATGG6250.031.5819842
ACATGGG6200.030.3206463
GTCCTAT804.75671E-429.3731291
ATACTGA650.00613377828.9212326
GGACCGC650.00613377828.9212326
GCTGTCC1005.2855674E-528.1982028
TCAACGC4150.027.178993
ATCAACG4050.026.6896552
CTGTCCT1606.809387E-826.4358169
AACGCAG4150.026.0465325
CAACGCA4500.025.065075
TATCAAC4550.024.7896292
ATGGGAT1351.4436369E-524.3688185
CGCAGAG4650.024.256527
Produced by FastQC (version 0.11.2)