FastQCFastQC Report
Sun 14 Apr 2019
SRR6576582_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576582_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447500
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11540.257877094972067No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7250.16201117318435754No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6420.143463687150838No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5990.13385474860335195No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5590.12491620111731844No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5520.12335195530726256No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5300.11843575418994413No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5260.11754189944134079No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5050.11284916201117319No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5020.11217877094972066No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5000.11173184357541899No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4870.10882681564245811No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4720.10547486033519553No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA4580.1023463687150838No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC500.001701979837.595223
GTATCAA13650.037.5725481
GTACATG28650.036.459031
TACATGG28550.036.2125552
ACATGGG28800.035.408693
CATGGGT7900.034.501944
ATGGGTA5000.031.9559365
TGTATAC600.0041608831.3293483
TCTAATA902.592665E-531.3293482
AACGCAG16050.031.040026
ATGGGAG4400.030.9733315
TGGGTAC5350.030.7471896
GGGTACC5400.030.459097
TCAACGC16850.029.5630054
CATGGGG13050.029.1687034
CAACGCA17250.028.8774875
ATCAACG17300.028.7940253
GGTACCT5800.028.3774878
GTATCAT856.7657704E-427.6775631
CTATTAT3650.027.070931
Produced by FastQC (version 0.11.2)