FastQCFastQC Report
Sun 14 Apr 2019
SRR6576580_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576580_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521441
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8780.16837954821350834No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6400.1227368005200972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14400.040.1722261
GTACATG34700.035.510361
TACATGG34650.035.2664532
ACATGGG35200.034.314853
CGGTTAA300.004161096631.33288870-71
ATGGGAG10300.030.1160745
TCAACGC19600.029.4944764
CATGGGA22500.029.4529114
ATCAACG19700.029.3447573
CAACGCA19750.029.270475
GCATAAG650.006131406428.9420911
CATGGGT6100.028.507794
AACGCAG20500.028.4288626
CATGGGG13100.026.9080124
ATAGACC700.00883229126.856763
TATCAAC22150.026.098952
ATGGGTA3850.025.6359985
GGTATCA8100.025.5476471
GGGTACC3300.024.2117777
ACGCAGA24200.024.08237
Produced by FastQC (version 0.11.2)