FastQCFastQC Report
Sun 14 Apr 2019
SRR6576579_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576579_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374422
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7170.19149515786999693No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4640.12392434205255032No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3820.10202391953464272No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3750.10015437127091892No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACT250.0052391256.3841364
GTATCAA9850.046.2774281
TACATGG22050.036.0173532
ACATGGG22250.035.9000133
GTACATG22400.035.4545821
AACGCAG13300.032.855426
ATCAACG13700.031.8961353
TCAACGC13900.031.4371994
CATGGGA12150.030.9377984
CAACGCA14450.030.5657925
GTCTATA804.778931E-429.3706591
CATGGGG8700.029.1642074
ACGCAGA15050.029.0350197
CGCAGAG15700.028.1322128
TATCAAC16450.027.1388452
CGACCGT350.00882607126.86214390-91
CATGGGT4600.026.5577434
GGTACCT3200.026.4300618
GCAGAGT17200.026.2251789
GGGTACC3050.026.1893527
Produced by FastQC (version 0.11.2)