FastQCFastQC Report
Sun 14 Apr 2019
SRR6576575_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576575_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643446
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14410.22395041697360774No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA10550.16396092290572947No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8790.13660820022193004No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7940.12339807847123146No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA7670.11920192215042133No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7600.11811402977095202No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG7330.11391787345014188No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG7300.11345163385894078No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT6970.10832299835572838No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG6920.10754593237039317No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6900.10723510597625907No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6850.10645803999092386No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6700.10412684203491825No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC6570.10210647047304668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13250.046.5369951
TACATGG30800.036.1630442
GTACATG31050.036.079311
TCAACGC17600.034.9804954
ATCAACG17600.034.713473
ACATGGG31900.034.4740643
AACGCAG18100.034.2738346
CAACGCA18200.033.8272935
CATGGGG14850.030.698184
GGTATCA6700.028.8039461
ACGCAGA22100.028.0682437
CGCAGAG22550.027.7337638
AGCGTTG700.00883621226.8552555
ATTAGTC1406.3648986E-726.8552553
CATGGGA14450.026.6694074
TATCAAC23350.026.3664552
GTATAAG1458.5292777E-725.9695781
ATGGGGG6350.025.1635885
GCAGAGT26300.023.4217439
CATGGGT9200.022.9875144
Produced by FastQC (version 0.11.2)