FastQCFastQC Report
Sun 14 Apr 2019
SRR6576573_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576573_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences240956
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6170.2560633476651339No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5920.2456880094291074No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4710.19547137236673914No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4390.18219093942462525No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4260.17679576354189147No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3850.159780208834808No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3720.15438503295207423No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3630.15064991118710472No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3520.14608476236325305No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3280.1361244376566676No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3140.13031424824449278No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3000.12450405883231792No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2920.12118395059678945No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2850.11827885589070204No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2840.11786384236126098No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2810.11661880177293779No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2730.11329869353740932No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC2530.10499842294858812No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAAAC200.00216211170.4900363
GTCCTAA301.3517376E-462.82731
GTATCAA10950.046.0446621
GGTACCT2200.040.6104558
ATGGGTA2500.039.4744225
GTACATG20500.038.156091
AACGCAG13200.037.3810846
GGGTACC2400.037.2030757
TACATGG21000.037.147132
ACATGGG22000.035.6722343
TGGGTAC2850.034.6266866
CATGGGG7750.034.562864
TCAACGC14600.034.440344
CATGGGT3700.034.2924544
GTCTTAC550.002678917334.2694361
CAACGCA14700.034.206055
ATCAACG14650.034.002023
ACGCAGA14850.033.2276277
CGCAGAG15350.032.1653188
ATGGGGG3550.031.7701595
Produced by FastQC (version 0.11.2)