FastQCFastQC Report
Sun 14 Apr 2019
SRR6576571_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576571_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186466
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4540.24347602243840702No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2630.14104447995881286No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2620.1405081891604904No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2470.13246382718565317No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2240.12012913882423605No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2090.11208477684939881No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2090.11208477684939881No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2040.1094033228577864No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2000.10725815966449648No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1930.10350412407623909No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG1930.10350412407623909No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAC250.00523467756.3850363
CATGGGT2500.041.349034
GTACATG11650.038.0248531
TACATGG12000.037.9917762
ACATGGG12000.037.198463
GCGTAGG550.002675671834.2738531
ACCGCGC550.002704033434.2002648
CGTAGGG550.002711160834.181922
GTATCAA6100.033.992921
ATGGGTA1950.033.734645
GGCTTAT702.1438682E-433.6618161
AACCGCG851.75012E-533.1676677
GGTACCT1606.002665E-1132.3299378
CATGGGG5000.031.9515234
GAATAGA600.004097813331.4176981
GTATATG753.2139942E-431.4176961
ATTATAC753.2701937E-431.3250183
ATGGGGA2000.030.5418955
ATGGGAC1102.9570092E-629.9011575
TGGGTAC2200.029.9011576
Produced by FastQC (version 0.11.2)