FastQCFastQC Report
Sun 14 Apr 2019
SRR6576564_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576564_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446567
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7930.17757693694339258No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5370.1202507126590187No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4470.10009696193404348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12100.043.6016431
ATCAACG14600.035.0810553
TCAACGC14750.034.72434
AACGCAG14900.034.6900946
CAACGCA15100.033.9194345
TACATGG24950.033.715542
CCTATAC702.1857898E-433.5637933
ACATGGG24750.033.034913
GTACATG25450.032.9459761
TATCAAC16550.031.5190162
CTATAGA1401.844819E-830.2820071
ACGCAGA17450.029.6207667
CTACGCA804.782624E-429.3683174
TAGACTA650.006157032728.9164965
CGCAGAG17750.028.8780388
TCTACGC856.8185804E-427.6407683
CATGGGA11700.026.9084054
CCGCTTA700.00880639926.8720979
CGATTGG350.00883996326.8540462-63
AACGCTG700.00884014326.8510345
Produced by FastQC (version 0.11.2)