FastQCFastQC Report
Sun 14 Apr 2019
SRR6576562_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576562_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432890
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8520.19681674328351312No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8020.18526646492180462No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5640.13028713992007207No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5280.12197093949964195No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4700.10857261660006005No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4460.10302848298643996No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4410.10187345515026913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10900.061.8026851
TAACGCA405.676315E-446.9940534
ATCAACG15500.043.35583
TCAACGC16000.042.2946434
CAACGCA16250.041.3547635
AACGCAG17250.038.957396
TATCAAC17450.038.2415772
GGTATCA3800.037.1907961
TACATGG25000.037.031312
GTACATG25400.036.7222441
ACATGGG26300.035.0221863
TATAACG550.002716831934.177492
ACGCAGA20450.032.8613627
CGCAGAG20600.032.644718
ATAGACG600.0041466231.35118
ATGGGCG1102.9657367E-629.9053045
CATGGGT7350.029.4112424
GCAGAGT23050.029.1748839
TAGACGA650.00613297828.9394769
GTAATAG1652.8812792E-928.5505071
Produced by FastQC (version 0.11.2)