FastQCFastQC Report
Sun 14 Apr 2019
SRR6576562_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576562_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432890
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7370.17025110305158353No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5510.12728406754602786No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4580.10580054979325003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11350.055.489741
CAACGCA15300.041.149695
ATCAACG15300.041.149693
GTACATG25900.040.1047941
TCAACGC15800.039.8474854
ACATGGG26350.039.762843
AACGCAG16050.039.2268076
TACATGG27050.038.2260252
TATCAAC17550.035.6187132
CATGGGT6750.035.4992874
CGCAGAG19100.032.962848
ACGCAGA19150.032.8767747
GGTATCA4600.032.6960951
CATGGGG11350.031.4608864
CATGGGA11500.031.4590844
ATAATAC1303.333207E-728.9134453
GCAGAGT22200.028.1482839
GGATAGT856.8224466E-427.6378548
ATCGCGA350.00884919826.84820238-39
ATGGGTA3800.025.9650345
Produced by FastQC (version 0.11.2)