FastQCFastQC Report
Sun 14 Apr 2019
SRR6576560_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576560_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349887
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7760.2217858908733391No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4640.13261424402735741No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3780.10803487983263169No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3740.10689165359101653No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3570.10203294206415213No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3570.10203294206415213No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAACG503.0021834E-547.007721
GTATCAA8050.044.3799631
TACATGG20000.039.251452
GTACATG20450.038.617591
ACATGGG20250.038.0541273
TGGGTAC3300.035.596646
CTATAAG1353.43789E-1034.8205341
AACGCAG10000.034.7707986
ATGGGTA3250.034.698515
ATCAACG10150.034.2569433
GGTACCT3350.033.6627358
TCAACGC10450.033.273494
CAACGCA10500.033.1150475
GGGTACC3350.032.260127
ATCCGGG600.0041537231.338481
CATGGGA10450.030.5756424
CATGGGG6700.029.4548914
CATGGGT6400.029.367234
TATCAAC12250.028.3965022
ACGCAGA12600.027.2229587
Produced by FastQC (version 0.11.2)