FastQCFastQC Report
Sun 14 Apr 2019
SRR6576559_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576559_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377859
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7840.20748480253216148No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4380.11591625447587592No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4150.10982932787097834No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4010.10612424211147543No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3880.1026838053347942No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCCG301.3701568E-462.6649935
GTATCAA9950.045.823931
TACATGG25250.040.024032
GTACATG25650.039.399871
ACATGGG25950.038.395893
CATGGGG9750.035.6708374
ATCAACG12850.034.7461553
TCAACGC12950.034.477844
AACGCAG13600.033.5211646
CAACGCA13500.033.4213265
TATCAAC14150.032.5546722
ATGGGAG6100.030.818855
CATGGGA13350.030.6283934
GGTTAAT953.742986E-529.6873461
ACGCAGA15700.029.037447
GTAGTAC650.00614981728.9223023
CGCAGAG16250.028.3438578
CATGGGT7000.028.1992454
GTATACG856.80336E-427.6499791
GCCCTAG700.00882410626.8599781
Produced by FastQC (version 0.11.2)