FastQCFastQC Report
Sun 14 Apr 2019
SRR6576554_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576554_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325312
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6850.2105670863663191No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3660.11250737753295298No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3450.10605203619909502No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACC405.671278E-446.9994
GTATCAA8050.046.1445241
TACATGG18300.039.826262
GTACATG18350.039.4615021
ACATGGG19000.038.0939253
GTACTAG1251.4915713E-1037.6165471
TACTAGG1500.037.6165472
ATCAACG9750.036.6351173
AACGCAG9800.035.9686246
TCAACGC9950.035.8987354
CAACGCA10050.035.5415345
GGGTACC2300.034.7383967
ACTAGGC1651.8189894E-1234.181093
GGTACCT2450.032.6115538
CATGGGT4950.032.282144
CATGGGA8800.031.5106934
ATGGGTA3000.031.3326665
TAGGCAT1751.70985E-1029.5422295
TATCAAC12350.029.316542
GTTATAC650.006148409628.9224623
Produced by FastQC (version 0.11.2)