FastQCFastQC Report
Sun 14 Apr 2019
SRR6576553_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576553_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349374
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8790.25159284892407563No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5920.1694459232799235No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4890.13996462243899088No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4750.1359574553343981No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4590.13137783578629206No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4360.12479463268588963No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4320.1236497277988631No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4180.11964256069427032No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4120.11792520336373057No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4030.11534916736792092No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3720.10647615449346545No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3700.10590370204995221No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3680.10533124960643894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11400.053.2901951
ATCAACG14600.041.520823
TCAACGC14650.041.3791124
GTACATG24500.041.327091
TACATGG24700.040.904452
CAACGCA14900.040.6848345
GGTATCA4550.040.3659931
AACGCAG15000.039.787036
ACATGGG25500.039.6211783
TATCAAC17450.034.7394832
CATGGGT5850.034.541544
ATGGGTA3550.034.4170845
ACGCAGA17450.033.9315877
CGCAGAG17600.033.6664928
GGGTACC2800.033.5661137
GACGGTA1052.0509851E-631.3508058
ACGGTAT1052.0509851E-631.3508059
ACGTGTG600.004160277531.3283717
TACGACG1052.0624775E-631.328375
GCAGAGT18950.031.019939
Produced by FastQC (version 0.11.2)