FastQCFastQC Report
Sun 14 Apr 2019
SRR6576553_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576553_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349374
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9130.261324540463801No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6170.1766015788238392No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5350.15313102863979575No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5110.1462615993176367No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4800.13738858644318125No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4680.1339538717821017No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4250.12164614424656672No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4110.11763897714197394No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4070.11649407225494741No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3660.10475879716292569No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3620.10361389227589919No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3600.10304143983238592No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3560.10189653494535943No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3520.1007516300583329No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3500.10017917761481965No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAACG250.005229748356.408852
CTAACGC250.00523567956.39273
TACGCTC250.00523864656.3846394
GTATCAA8950.055.1404231
TGCCCGC601.601753E-647.000651
GTACATG24000.042.1047441
TACATGG23950.042.002412
ACATGGG24100.041.144053
ATCAACG12350.039.5738223
TCAACGC12550.038.568714
CAACGCA12750.037.9637155
CATGGGA13400.036.8235934
AACGCAG13150.036.8089266
CGCCTAC550.002716155734.177397
ATGGGAG5400.033.9400525
TATCAAC15050.032.795842
ACGCAGA14950.032.691427
CGCAGAG15250.032.0483138
CATGGGG7400.031.7526454
GACCGTG753.273395E-431.3292777
Produced by FastQC (version 0.11.2)