FastQCFastQC Report
Sun 14 Apr 2019
SRR6576552_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576552_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379865
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9400.24745633317099494No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5790.1524225711766022No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5710.1503165598304661No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4970.1308359548787069No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4770.1255709265133666No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4690.12346491516723047No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4420.11635712687402104No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4370.11504086978268596No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4220.11109209850868071No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4220.11109209850868071No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4070.10714332723467548No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3910.10293130454240322No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCCG250.005234568456.39668394
GTATCAA11100.054.202151
GGATAGC652.7811038E-643.3820658
ATCAACG15150.038.7765963
GTCTTAC500.001699985137.6027371
TCAACGC15500.037.5977864
TACATGG27700.036.8221742
CAACGCA16050.036.309395
GTACATG28500.036.2833441
ACATGGG28100.036.1259883
AACGCAG16200.035.9731946
CTAGTAC702.1830133E-433.5694543
TATCAAC18050.033.332072
ACGCAGA18050.032.286197
CGCAGAG18500.031.7548898
ATGGGAT6050.031.0725525
CATGGGG9000.029.7649154
CATGGGT6600.029.1952534
GCAGAGT20250.029.0106379
GTAGTAA650.006146863628.9251841
Produced by FastQC (version 0.11.2)