FastQCFastQC Report
Sun 14 Apr 2019
SRR6576548_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576548_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400141
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6940.1734388628008627No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4250.10621256007257443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11000.040.173091
GTACATG22950.033.184281
TACATGG23150.033.1006622
ATCAACG13400.032.969673
TATACCT1001.4082798E-632.8995174
ACATGGG23150.032.889373
TCAACGC13800.032.0140234
AACGCAG13400.031.9174446
CAACGCA13950.031.6697865
CATGGGG7950.031.3328744
ATGGGGA5200.028.018825
TATCAAC15800.027.9686072
ACGCAGA15300.027.9538387
GTAATAC1205.8313744E-627.4162643
TACCGTC1755.5315468E-926.856757
CGCAGAG16350.026.158648
CATGGGA13950.025.9422724
TATACTG1659.287214E-825.6359885
ATGGGAG6350.025.1649865
ACCGTCG1701.239805E-724.8819898
Produced by FastQC (version 0.11.2)