FastQCFastQC Report
Sun 14 Apr 2019
SRR6576547_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576547_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260831
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7120.2729736879435343No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4960.19016144553369807No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4670.17904313521015525No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3920.15028888437340654No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3890.14913871433993658No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3260.12498514363706767No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3230.12383497360359773No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3140.12038446350318788No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3120.11961768348087459No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3040.11655056339162138No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2980.1142502233246815No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2880.11041632321311501No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2820.10811598314617511No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2690.10313191300113866No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2660.10198174296766871No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2610.10006479291188547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9400.041.1539841
CAACGCA12300.031.3243875
CGTACGA300.00416473431.32438710-11
ATCAACG12400.031.071773
TCAACGC12600.030.9514774
AACGCAG12850.029.9836546
GTACATG18800.029.3598541
ACATGGG18850.028.4162883
CATGGGG6050.027.9589564
ATGGGAT1354.623107E-727.8438995
TACATGG19700.027.677842
AACCCTT1555.0658855E-827.282536
GCTTAGA700.00866382726.958011
ACGCAGA14350.026.8494747
ACACGCT2650.026.5961769
ACCCTTG1606.892151E-826.429957
CATGGGT4150.026.0407544
CGCAGAG15000.025.6859978
GCACACG2800.025.1713817
TATCAAC15350.025.1099512
Produced by FastQC (version 0.11.2)