FastQCFastQC Report
Sun 14 Apr 2019
SRR6576547_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576547_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260831
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7110.27259029793237766No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3820.14645498426184006No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3640.13955396406102036No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3510.1345698939159839No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3340.12805226372632086No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3230.12383497360359773No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3230.12383497360359773No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3170.12153463353665785No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3070.11770073342509135No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2990.11463361333583814No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2990.11463361333583814No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2680.10274852298998202No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2630.10083157293419877No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACG200.002156591770.5371251
GGACAAT503.0038498E-546.99776
AGACGGC608.807866E-539.1647456
GTATCAA8850.038.7887731
ATGGGTA2250.037.598165
CATGGGT3200.035.2482764
GCACCGT550.002713827934.1801456
GTACATG15650.033.953971
GGTCAAC1001.4061661E-632.8983887
GGTACCT2150.032.7890938
TACATGG16400.032.382562
TCAACGC9850.031.9679784
ACATGGG16500.031.6166343
ATCAACG10000.031.4884593
TGCCCGC753.2585772E-431.349831
TAGGACA753.269594E-431.33184
TATAAGC753.269594E-431.33182
CAACGCA10300.030.5713185
GGGTACC2350.029.9985337
TAGGTCA1102.9579915E-629.9076275
Produced by FastQC (version 0.11.2)