FastQCFastQC Report
Sun 14 Apr 2019
SRR6576545_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576545_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344593
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10290.29861314652358No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6060.17585963731126286No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5800.16831450435731427No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5520.1601889765607543No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5320.15438502813464No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5080.14742029002330284No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4920.14277713128241143No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4690.13610259059238No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4580.13291041895801714No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3990.11578877110097999No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3740.10853383556833714No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3680.10679265104050287No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3640.10563186135528001No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3630.10534166393397427No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3630.10534166393397427No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3590.10418087424875142No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3490.10127890003569429No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7500.055.7733351
GTACATG22000.042.0863651
TACATGG22450.041.2427642
ACATGGG22800.040.403513
CATGGGG8100.038.876544
ATCAACG11200.037.7678573
AACGCAG11350.037.682826
CAACGCA11400.037.1052635
TCAACGC11450.036.9432334
ATGGGTA4050.034.8148165
CATGGGT6150.034.3902444
ATGGGGG3250.031.8153865
GGTACCT3600.031.3333348
ACGCAGA13650.030.989017
CATGGGA10500.030.8857154
TCTTAAC1102.9604998E-629.909092
TCTATAC1102.9604998E-629.909093
ATGGGAT3950.029.7468385
GGGTACC3800.029.6842127
CCTTTAT953.7447375E-529.6842121
Produced by FastQC (version 0.11.2)