FastQCFastQC Report
Sun 14 Apr 2019
SRR6576540_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576540_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254057
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4890.19247649149600285No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2970.11690289974297105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA5450.063.7978061
ATCAACG7100.047.6480033
TCAACGC7250.047.310274
AACGCAG7350.046.666596
CAACGCA7400.046.3512765
TACACCG450.001013924841.765535
ACGCAGA8950.038.323967
TATCAAC8850.037.695162
CGGCGCA500.001701982337.58897894
CGCAGAG9150.037.4862758
TACATGG16400.036.3856742
GTACATG16600.036.230341
GTCTAGA651.4128274E-436.143251
ACATGGG16950.036.0366333
GCAGAGT9700.034.876379
TCTAAGC959.4338066E-734.6214263
ATGGGCG550.002716946634.17185
CTAAGCT702.1839907E-433.561594
CATGGGA7750.032.1325154
GTGTAAG600.004160904331.3241481
Produced by FastQC (version 0.11.2)