FastQCFastQC Report
Sun 14 Apr 2019
SRR6576539_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576539_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279739
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5410.19339455706926814No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3430.12261429403837149No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3300.11796710505149442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6500.042.1017231
GTACATG17250.041.3021931
ACATGGG16900.040.8794023
TACATGG17300.040.74922
TATAGGG500.001700287337.5979232
ATCAACG7850.035.322893
TCAACGC7950.034.878584
CATGGGA9050.034.2743534
CAACGCA8250.033.6102685
AACGCAG8300.032.841566
CATGGGG6950.031.7824174
ATGGGCG753.2703154E-431.3316065
ATGGGGG2500.030.0783425
ACGCAGA9250.029.4686437
CGCAGAG9350.029.1534738
TATCAAC9850.029.1049942
GGGATAC650.006030709529.0356711
GTGATAA856.6502625E-427.7546841
CATGGGT3800.027.2090264
CTATTAG700.008659109526.9616971
Produced by FastQC (version 0.11.2)