FastQCFastQC Report
Sun 14 Apr 2019
SRR6576539_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576539_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279739
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4790.17123104036262374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6350.048.858291
TATCACG450.001011833341.7845342
GGTATCA3150.038.7999271
GTACATG17550.034.5525931
TACATGG17450.034.2118342
ATCAACG8750.033.839423
TCAACGC8750.033.839424
CAACGCA8800.033.6471525
AACGCAG9000.032.8994376
ACATGGG17850.032.64933
TATCAAC9850.030.5430092
ACGCAGA10700.027.6724247
TAGTAGC700.00882553926.8566846
ATGGGGG3350.026.656265
CGCAGAG11150.026.55568
CATGGGT4750.025.7258744
CATGGGG7350.025.5777934
GCAGAGT12100.024.8590779
CATGGGA8150.024.7971214
AACACTC2151.8644641E-924.04617
Produced by FastQC (version 0.11.2)