FastQCFastQC Report
Sun 14 Apr 2019
SRR6576538_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576538_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187225
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3570.19067966350647617No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2100.11216450794498597No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1890.1009480571504874No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACCG250.005234569356.3854185
GTTATAT601.6007398E-646.9878461
GTATAGC450.001013067841.7669751
TATACTG500.001700549237.590285
GTATCAA5950.037.1164471
AACGCAG6550.034.4338466
ATGGGGA2600.034.3372765
TATTAAG550.002714669334.1729772
TACATGG10250.033.4645162
CAACGCA6800.032.4768945
ACATGGG10600.032.3595543
TCAACGC7000.032.2202384
GTACATG10800.032.1953771
ATCAACG6900.032.0062143
CGCAGAG7150.031.5442898
TATGGGT600.004157429631.3252324
ATTTCCG452.593871E-531.32523268-69
ACGCAGA7250.031.1091967
TATCAAC7550.029.873072
CATGGGG4700.028.9925044
Produced by FastQC (version 0.11.2)