FastQCFastQC Report
Sun 14 Apr 2019
SRR6576531_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576531_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239842
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4800.20013175340432451No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3030.12633316933647984No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2930.12216375780722309No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2750.11465881705456091No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6250.043.682481
GTACATG14450.037.7876131
ACATGGG14300.036.80583
TACATGG15150.035.6713452
TATAGGT702.1817446E-433.5665174
AACGCAG8000.033.4825976
CAACGCA8300.032.2723855
ATCAACG8450.031.6995013
TCAACGC8450.031.6995014
ATATAGG902.5880277E-531.3287473
TATCAAC9200.030.6476842
CATGGGG5250.030.4336384
CATGGGT2850.029.6798654
CCGGGGT650.006148990728.9188424
GTATAAT1005.2514042E-528.2429811
CATGGGC2500.028.1958714
ACGCAGA9600.027.9021667
CGCAGAG9750.027.0078148
CCATAGA700.00875702426.8980791
TACTAGG700.00882863626.8532122
Produced by FastQC (version 0.11.2)