FastQCFastQC Report
Sun 14 Apr 2019
SRR6576531_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576531_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239842
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5120.21347387029794612No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2870.11966211088966905No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2820.11757740512504065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6000.047.7812161
GTATAGG555.271926E-542.7253761
CTATGGT952.1096639E-839.568944
ATCAACG7300.038.620373
TCAACGC7450.037.8427774
CAACGCA7700.037.224355
AACGCAG7950.036.0537766
TATCAAC8550.032.9809952
GGTACCT1755.456968E-1232.220428
TACATGG14850.031.6484262
GTACATG15100.031.435691
CCCTTAC753.2687248E-431.3319441
ACATGGG15000.031.0121573
CATGGGT3800.029.6767064
GTATAAA1303.3143442E-728.9217931
ACGCAGA9500.028.687487
TATGGTC1154.1864478E-628.6014615
CGCAGAG9600.028.3886538
GGGTACC2053.6379788E-1127.5052387
TATAGGG700.00882424426.8559512
Produced by FastQC (version 0.11.2)