FastQCFastQC Report
Sun 14 Apr 2019
SRR6576529_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576529_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317594
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7760.24433710964312927No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4520.14232006901893612No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4070.1281510355989093No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3850.12122395259356285No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3710.11681580886288784No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAAA405.6794763E-446.9848865
CCCGCTA405.6794763E-446.9848863
GATTCGC450.001014450441.7643439
GTATCAA10800.040.926471
AACGCAG12600.034.6793176
TGGGACG959.4457937E-734.620446
CCGCTAA550.00271834634.1708264
ATCAACG12900.033.872823
GTACATG19600.033.826981
TACATGG19650.033.740912
TCAACGC13050.033.4834824
CAACGCA13100.033.3556825
ACATGGG19650.033.2361263
CATGGGT4900.031.642884
CGCAGAG13900.031.4359328
CTAGGCG600.004163037531.3232579
ACGCAGA14000.031.2113887
GTCTAGA804.7601722E-429.388691
TATCAAC14950.029.2510832
GTATAGG650.00613357128.9365561
Produced by FastQC (version 0.11.2)