FastQCFastQC Report
Sun 14 Apr 2019
SRR6576528_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576528_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358561
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6680.18630023901093537No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4070.11350927736145315No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4070.11350927736145315No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3890.10848921104079919No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3740.10430582244025424No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGAC352.9351882E-453.7127723
GTATCAA9250.047.3782541
TCAACGC11500.038.0076264
AACGCAG11550.037.843096
ATCAACG11550.037.4361763
CAACGCA11900.036.7300575
GTACATG19450.035.6152151
TACATGG19350.035.4615332
ACATGGG19050.035.2798423
CGACGGT801.1566533E-535.249017
CATGGGA9150.032.8733864
ACGCAGA13650.032.3653877
TATCAAC14600.031.2251472
CGCAGAG14050.031.1094448
CATGGGT4850.030.0403924
TACGACG953.7458783E-529.6833745
CTACGAC1005.3144868E-528.1992054
ACGGTAT856.808001E-427.646289
GGGTACC2600.027.114627
GCAGAGT16200.026.9807199
Produced by FastQC (version 0.11.2)