FastQCFastQC Report
Sun 14 Apr 2019
SRR6576528_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576528_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358561
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7510.20944832260061746No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4260.11880823625547675No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3940.10988367390764751No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3850.10737364074732055No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3800.10597917788047222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8800.042.7272721
GTACATG19550.039.18671
GTATAAT1700.038.7058831
TACATGG19700.037.934012
GTATTGA500.00170046637.61
ACATGGG20500.035.5365873
CATGGGT4900.034.5306134
TATACGG550.002714527534.1818162
ATGGGTA2950.033.4576265
ATCAACG11400.031.333333
TGGGTAC3050.030.8196746
CATGGGG8900.030.6292154
TCAACGC11750.030.44
GGGTACC2950.030.2711857
CAACGCA12300.029.040655
AACGCAG12750.028.7529416
TACTAAT856.806879E-427.647062
GGTACCT3300.027.0606088
CATGGGA9400.027.04
ATGGGGA4700.027.05
Produced by FastQC (version 0.11.2)