FastQCFastQC Report
Sun 14 Apr 2019
SRR6576527_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576527_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences240231
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9030.3758882076001848No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5690.23685536004928587No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4670.19439622696487963No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4620.19231489691172246No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4040.1681714682950993No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3930.16359254217815353No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3790.15776481802931344No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3750.15609975398678771No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3490.14527683771037042No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3320.13820031552963605No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3300.1373677835083732No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3170.13195632537016455No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3110.12945872930637595No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3040.1265448672319559No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2720.11322435489175002No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC2690.11197555685985572No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2670.11114302483859287No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2650.11031049281732999No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT2590.10781289675354137No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCG2520.10489903467912134No Hit
CTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG2430.10115264058343844No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2430.10115264058343844No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2420.100736374572807No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGT503.0058047E-546.990634
GTACATG14700.038.0955471
ACTACGC500.001701013137.5925063
TTAGTGC500.001701013137.5925066
TACATGG14950.037.4039152
GTATCAA6950.037.2410961
ACATGGG15200.036.788723
ATTAGTG651.4118185E-436.146645
CATGGGG7450.035.9525644
CTACGCT550.002715404134.1750074
GGAGAGT702.1824337E-433.564746
TACTACG702.1824337E-433.564742
AAGTCGG600.00414162131.35319594
GGTAAGG902.5779462E-531.3466669
TTATTAG600.00415854631.3270873
TATCCCC600.00415854631.3270875
TACTCCC1859.094947E-1230.4804125
GGTACCT2800.030.2271428
ATGGGAG1751.70985E-1029.536975
TAAAGGT804.775331E-429.3691444
Produced by FastQC (version 0.11.2)