FastQCFastQC Report
Sun 14 Apr 2019
SRR6576527_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576527_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences240231
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9160.38129966573839347No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5610.23352523196423441No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4750.1977263550499311No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4420.18398957669909377No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4100.1706690643588879No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3750.15609975398678771No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3560.14819069978479046No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3390.1411141776040561No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3360.13986537957216177No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3220.13403765542332172No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3020.12571233521069303No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2980.1240472711681673No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2980.1240472711681673No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2910.12113340909374727No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2840.11821954701932724No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG2810.11697074898743293No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2720.11322435489175002No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT2710.1128080888811186No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2690.11197555685985572No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG2690.11197555685985572No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC2590.10781289675354137No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG2460.10240143861533274No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTAG250.00523807956.3807685
GTATAGT602.5514964E-854.8146328
CCGAACT352.93777E-453.695971
GTCTTAG1050.053.695971
TTAGGTA601.6035629E-646.983974
CGAACTC405.677241E-446.983972
GTCGTCC405.677241E-446.983978
CTTAGGT601.6035629E-646.983973
GTATCAA6250.046.6080971
TATAGTA751.4784018E-743.8517079
TAGGTAT652.7822098E-643.369825
GGTATAG652.7822098E-643.369827
TACGCTG555.2811938E-542.71275
TCGTCCC450.001014053241.763539
TACATGG14000.041.6143762
GTACATG14150.041.1732331
ACATGGG14650.039.4472963
CTACGCT608.8209534E-539.153314
ACTACGC608.8209534E-539.153313
GTGTAGA608.8209534E-539.153311
Produced by FastQC (version 0.11.2)