FastQCFastQC Report
Sun 14 Apr 2019
SRR6576526_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576526_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264767
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8480.32028160609139356No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5790.21868284189494913No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4320.16316232763146465No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4280.16165156533858072No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4200.15863004075281287No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3670.13861244037210074No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3620.13672398750599585No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3560.13445784406666994No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3460.13068093833446012No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3410.1287924854683552No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3340.12614865145580834No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3100.11708407769850473No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3030.11444024368595783No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG2870.10839719451442212No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2860.10801950394120113No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2690.10159876419644441No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2680.10122107362322344No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2680.10122107362322344No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2680.10122107362322344No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAC250.005224241456.4200631
TAGGCGG352.9336946E-453.71315
CTTAAGT405.6693784E-446.9989623
GTATCAA5650.043.2720221
TACATGG13900.040.5899772
TTAGGTA608.806834E-539.1658024
CATGGGT3600.039.1658024
GTACATG14350.038.9894751
CTAGGCG500.001699847237.599174
AACGCAG6750.036.2066046
ATGGGTA2600.036.153055
TAGGTAT651.4107373E-436.153055
ACATGGG15350.036.1294943
GGGTACC2350.035.9992077
GGTACCT2450.034.5298468
CAACGCA6950.034.4884455
TCAACGC7100.034.4217764
GCCTTAA550.002708520534.1939771
CTTAACC550.002713543834.1810653
TGAACCG550.002713543834.1810655
Produced by FastQC (version 0.11.2)