FastQCFastQC Report
Sun 14 Apr 2019
SRR6576525_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576525_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370001
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5340.14432393425963713No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4460.12054021475617634No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC250.005233569556.399113
GTATCAA7700.053.1605261
ATCAACG9650.039.937193
CAACGCA9850.039.1262865
TCAACGC9950.038.7330554
AACGCAG10700.036.457376
GTATACG550.002700485834.218271
TATACGG550.002709455634.195142
TATCAAC11900.032.794292
GTACATG16350.031.3667491
CCGAACT753.2508947E-431.3667491
GGTATCA3400.030.4441951
ACGCAGA13100.029.7781547
TACATGG17150.029.6092012
ACATGGG17450.029.0883663
CGCAGAG13600.029.0289528
CATGGGT3350.028.059264
GTATAGC1406.289865E-726.8857821
GATCGCG459.5153425E-426.11069776-77
GCAGAGT15000.026.0062569
Produced by FastQC (version 0.11.2)