FastQCFastQC Report
Sun 14 Apr 2019
SRR6576520_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576520_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556547
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9780.1757263986689354No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8860.159195898998647No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7310.1313456006410959No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6130.1101434380205086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13450.055.292451
ATCAACG16300.045.550973
TCAACGC17150.043.567354
CAACGCA17400.042.941385
AACGCAG17650.042.3331456
GGTATCA4550.040.3444941
GTACATG28450.038.879141
TACATGG28600.038.612682
ACATGGG28850.038.278083
TATCAAC20950.035.6649172
CGCAGAG20850.035.4106568
ACGCAGA20900.035.3005527
CATGGGT8100.033.0687644
ATGGGTA4800.032.3073125
GCAGAGT23700.031.5492579
CGTATAG953.7117134E-529.727521
ATGGGAG5950.029.2221985
CACGCAA650.006134714528.9392369
CATGGGA13300.028.6194654
CATGGGG11750.027.1956334
Produced by FastQC (version 0.11.2)