FastQCFastQC Report
Sun 14 Apr 2019
SRR6576519_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576519_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606302
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10540.17384075922560044No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7410.1222163212392504No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6680.11017611685265759No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6490.10704236502601014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12550.057.686921
GGTATCA4650.042.461631
ATCAACG17800.040.3984153
TCAACGC18000.039.9495474
CAACGCA18350.039.6998185
AACGCAG19150.037.7959146
GTACATG31050.035.277251
ACATGGG31750.035.0830653
TACATGG31750.034.499482
TAACGCA702.1839353E-433.5710454
CATGGGA12850.033.283674
ACGCAGA22600.032.2341467
CGCAGAG23050.031.4009448
TATCAAC22900.031.203872
CCGAACT650.006144816528.9299031
CATGGGT7800.028.320194
TAACGCC700.00883327926.8568364
CATGGGG13700.026.758824
GCAGAGT27100.026.7081839
AGAGTAC25050.024.76618410-11
Produced by FastQC (version 0.11.2)