FastQCFastQC Report
Sun 14 Apr 2019
SRR6576513_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576513_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167955
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4280.2548301628412372No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3050.18159626090321812No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2800.16671132148492154No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2620.15599416510374803No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2490.1482539966062338No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2370.14110922568545145No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2290.13634604507159656No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2280.13575064749486468No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2160.12860587657408235No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2050.12205650323003185No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG1980.11788872019290883No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT1970.11729332261617696No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG1950.11610252746271323No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT1780.10598076865827157No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1770.1053853710815397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG14300.041.8025781
GTATCAA8150.041.5824661
TACATGG14950.040.2279322
ATGGGAG2500.039.467375
ACATGGG15100.038.583683
AACGCAG8800.038.4422456
GTATATG500.001685686437.6552351
ATGGGAT2150.037.1509065
CATGGGT2200.036.3065644
ATCAACG9300.035.8702473
TCAACGC9550.034.439244
CAACGCA9600.034.2598725
TAATACC550.002714692834.1708874
CGCAGAG9900.033.7264148
CATGGGA8750.033.2922064
ACGCAGA10200.032.705227
GCAGAGT10700.031.6443149
GACATAT600.004121229531.3793641
CGGCAAA300.0041451731.35131370-71
GGGAGAC753.2698674E-431.323317
Produced by FastQC (version 0.11.2)