FastQCFastQC Report
Sun 14 Apr 2019
SRR6576512_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576512_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184459
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4180.22660862305444568No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3280.17781729273171815No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3260.17673304094676867No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3020.16372201952737464No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2970.1610113900650009No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2810.15233737578540488No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2430.13173659187136436No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2420.13119446597888962No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2310.12523108116166737No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2220.1203519481293946No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2180.1181834445594956No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2180.1181834445594956No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG1970.10679880081752584No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1860.10083541600030359No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8000.043.5312421
GTGTAAG555.2257536E-542.7825471
GTCTATG608.728512E-539.217341
TTAAGAC500.001698855237.5976143
ATCCCCT651.4094214E-436.1515546
GTACATG14650.035.9782261
ACATGGG14850.034.8126073
TACATGG15100.034.5474782
AACGCAG9700.034.399886
CATGGGG5750.034.3282554
ATCAACG10200.033.1743663
TCAACGC10300.032.8522834
CAACGCA10400.032.0845035
GTCCTAT1052.0323423E-631.3738691
CATGGGT2150.030.602714
TATCAAC11400.030.0945822
GCTCTTA804.729555E-429.4130021
ATACTCC804.7672866E-429.3731374
CGCAGAG11400.029.2700718
ACGCAGA11450.029.1422567
Produced by FastQC (version 0.11.2)