FastQCFastQC Report
Sun 14 Apr 2019
SRR6576512_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576512_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184459
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5440.29491648550626426No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3020.16372201952737464No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3000.16263776774242514No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2970.1610113900650009No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2570.13932635436601087No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2500.13553147311868763No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2430.13173659187136436No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2380.1290259624089906No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2210.11980982223691987No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2160.11709919277454611No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2020.1095094302798996No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1970.10679880081752584No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC1960.1062566749250511No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG1910.10354604546267734No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT1870.10137754189277835No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGCA250.005229093656.3999983
ATGGGTA1650.054.1212165
GGTACCT1750.051.0285728
GGGTACC1750.048.3428577
CATGGGT2800.043.6428534
ACGGCCC450.001011740641.777788
GTACCTG2150.041.5348859
GTACATG15200.041.1251
TGGGTAC1950.040.9743586
TACATGG15300.040.856212
ACATGGG15150.040.6402663
GTATCAA5700.039.578951
ATGGGGG2200.038.4545445
ATGGGAG3100.034.8709685
CATGGGG5450.034.495414
CTACACC1405.0386006E-1033.5714264
CATGGGC2550.031.3333344
ATGGGAT1351.3144017E-831.3333345
CGGCTCG1052.052966E-631.3333328
CATGGGA8100.029.0123444
Produced by FastQC (version 0.11.2)