FastQCFastQC Report
Sun 14 Apr 2019
SRR6576511_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576511_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235139
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4700.19988177205822938No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2850.12120490433318164No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2810.1195037828688563No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2770.11780266140453094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAACG602.5407644E-854.843141
CAAACGC558.7699846E-751.2818952
ATGGGTA2650.039.0092475
TACATGG15500.036.0903242
CATGGGT4350.035.646384
GTACATG16050.034.8535841
ACATGGG16350.034.7742963
GTATCAA6700.034.379281
AAACGCA851.7518318E-533.168293
TCTTGCG902.582064E-531.3389362
CGCCGGT902.5895859E-531.3256077
CGCAAAA953.746031E-529.6768916
CATGGGG6050.029.5133824
GGTACCT2750.029.0473798
GTATCCA1503.6650817E-828.2050421
ACGCAAA1005.3147178E-528.1930455
ATCAACG8350.027.0113013
TCAACGC8550.026.929034
GGGTACC2800.026.850527
GGAGTGC1057.409347E-526.850526
Produced by FastQC (version 0.11.2)