FastQCFastQC Report
Sun 14 Apr 2019
SRR6576510_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576510_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254269
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4440.17461821928744756No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2920.11483900908093396No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2740.1077598920827942No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2550.10028749080698No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGC250.005232968456.395637
GTACATG15550.040.6263051
TACATGG15800.039.2627832
ACATGGG15800.038.6678923
GTATCAA5850.037.876941
ATTGACT1001.4062934E-632.897454
CATGGGT3950.032.1240964
ATGGGTA2650.030.1486075
CATGGGA7600.029.6819114
ATCAACG7600.029.063542
GGTACCT2500.028.1978178
CAACGCA8250.027.912995
CATGGGG5950.027.6449184
TCAACGC8400.027.4145434
GTATAAA1554.9023583E-827.3743461
AACGCAG8500.027.092026
ATCTAAT700.00869198326.939831
TCGATAA350.00882633626.86034666-67
TGGGTAC2650.026.6017136
ATGGGAT2550.025.8019245
Produced by FastQC (version 0.11.2)