FastQCFastQC Report
Sun 14 Apr 2019
SRR6576510_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576510_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254269
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4760.18720331617302935No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3230.12703082168884133No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3150.1238845474674459No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2850.11208601913721294No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2610.10264719647302659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7000.046.3355521
TACATGG15850.039.4444272
GTACATG16350.038.2381741
ATCAACG8300.037.9380153
TCAACGC8400.037.486374
ACATGGG16550.037.2007033
CAACGCA8500.037.0453535
AACGCAG8750.035.9869166
CATGGGG5450.034.4938284
ATGGGGC1107.659219E-834.180255
CATGGGT4950.033.2307934
ATGGGTA2600.032.5369645
TACTATG1052.0545958E-631.3380532
ACGCAGA9950.031.1744427
TATCAAC10500.030.8903682
CGCAGAG10250.030.262028
GTTATAG804.7718227E-429.3736483
GATATGC650.006140757428.9274351
CACACCG650.006146662428.9217475
CATGGGA8400.028.53444
Produced by FastQC (version 0.11.2)