FastQCFastQC Report
Sun 14 Apr 2019
SRR6576509_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576509_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150342
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG2490.16562238097138524No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1690.11241037102073938No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT200.00214678370.603461
TACCGGG200.00216101170.486032
GTATCAA2600.063.362081
CTACCGG301.3574133E-462.758641
ATAGCGT250.005231316756.388826
CTAGTAC352.932154E-453.7036443
TCAACGC3350.047.692044
ATCAACG3500.045.6480983
CAACGCA3700.043.1806345
AACGCAG3850.041.498276
TATCAAC3900.040.966242
GTCTACT500.001685133937.6551781
TCCTACC500.001698978437.592552
CGCAGAG4550.035.1256038
ACGCAGA4750.033.6354377
GTACATG7250.033.110591
CATGGGG2850.032.9759184
TACATGG7350.032.605782
ACATGGG7600.031.5332243
CACCGAC300.004160173231.32712430-31
Produced by FastQC (version 0.11.2)