FastQCFastQC Report
Sun 14 Apr 2019
SRR6576508_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576508_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184973
Sequences flagged as poor quality0
Sequence length100
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG2000.10812388835127289No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA3200.049.936071
ATTCCGA250.001698518237.60908576-77
TACATGG10850.036.8192182
ATCAACG4400.036.317143
ACATGGG11300.035.7688873
GTACATG11200.035.668621
TCAACGC4500.035.5100864
CAACGCA4850.033.9165535
AACGCAG5050.033.5039866
CATGGGT3050.032.3597264
TATCAAC5000.031.959082
GGTATCA1807.2759576E-1231.3324321
GTAATAG600.004152627731.332431
CATGGGA5550.030.485614
GTCCTAG804.7663672E-429.3741571
ATATAAG804.7663672E-429.3741573
CGGACCA804.7663672E-429.3741574
ATGGGAG2900.029.1715765
GCTTAGT650.006141871728.9222451
GCCCTAA650.006141871728.9222451
Produced by FastQC (version 0.11.2)