FastQCFastQC Report
Sun 14 Apr 2019
SRR6576507_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576507_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318031
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6350.19966607028874545No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4550.1430678141439042No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3880.12200068546776886No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3520.11068103423880062No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3280.10313460008615513No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3270.1028201653297949No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3220.10124799154799374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10950.046.8597071
ATCAACG13150.038.9525683
TCAACGC13350.038.369014
CAACGCA13400.038.225845
AACGCAG13450.037.734356
TACATGG21950.034.6829382
GTACATG22350.034.5424771
ACATGGG21900.034.1183783
GTCTTAC851.7318906E-533.2291681
TATCAAC15700.033.2245182
CATGGGT5600.032.7274364
ACGCAGA15650.032.4298367
ATGGGTA3350.032.2640155
CGCAGAG15900.032.2357678
ACTTCGT600.004146634631.3485458
GCAGAGT17850.028.7142129
CATGGGA11250.028.4048024
ATGGGAG3900.027.713965
AATAGAC700.00883278226.853283
CATGGGG8000.026.4336994
Produced by FastQC (version 0.11.2)