FastQCFastQC Report
Sun 14 Apr 2019
SRR6576503_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576503_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314551
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7960.2530591223680738No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5240.1665866584433049No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4570.1452864559324243No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4400.13988192693712626No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3890.12366833995123207No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3730.11858172442624566No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3680.11699215707468742No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3490.11095180113876604No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3300.10491144520284469No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3300.10491144520284469No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3210.10205022397003984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3700.047.1018031
GTATCAA7800.047.10181
TCAACGC9950.036.3695224
CAACGCA10100.035.829385
AACGCAG10200.035.478116
CATAGGT801.1564882E-535.2477384
ATCAACG10300.035.1336633
TTGACGG959.4264215E-734.62935394
GTCCTAT1151.11152985E-732.766471
GTACATG14600.032.261511
TACATGG14800.031.7698652
GTCTTAA902.5499865E-531.40121
CGTTATT902.580693E-531.3462682
ACGTTAC300.00416087131.33132234-35
CTTAAGT902.5891219E-531.331323
GCGTTAT953.688818E-529.7485051
ACCTAAG953.688818E-529.7485051
ACATGGG15650.029.729723
TATCAAC12450.029.457942
ACGCAGA12300.029.4208747
Produced by FastQC (version 0.11.2)